T7

Part:BBa_K3398001:Design

Designed by: MingXiao Wei   Group: iGEM20_ZJU-China   (2020-10-08)


Enhanced T7 promoter 2


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 59
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We chose these sites for mutation with the help of previous research


Source

It is derived from BBa_I712074 with some point mutation.

References